Enzyme-linked immunosorbent assay for detecting antibodies to Brucella abortus in bovine milk and serum

Am J Vet Res. 1980 Dec;41(12):2082-4.


An enzyme-linked immunosorbent assay (ELISA) method was evaluated for the detection of antibodies to Brucella abortus in cows milk. Milk samples from seropositive or -negative cows were sed to determine the distribution of absorbance values to classify milk as ELISA positive or ELISA negative. Brucella abortus was isolated from milk samples from 10 (45%) of the 22 cows whose milk and serum were ELISA positive. The ELISA was evaluated and determined to be an appropriate method for detecting antibodies to B abortus in bovine milk.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Bacterial / analysis*
  • Brucella abortus / immunology*
  • Brucellosis, Bovine / diagnosis*
  • Cattle / immunology
  • Enzyme-Linked Immunosorbent Assay*
  • Female
  • Immunoenzyme Techniques*
  • Milk / immunology*


  • Antibodies, Bacterial