Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes

Nature. 1981 Apr 16;290(5807):562-5. doi: 10.1038/290562a0.


A putative diversity segment of immunoglobulin heavy-chain genes (D segments) has been identified 700 base pairs 5' to JH1 DNA on the germ-line genome of the mouse. This 10-base pair D segment is flanked by two sets of sequences related to (SEE FORMULAR IN TEXT) which are possible recognition sites for a recombinase. The spacer separating the heptamer and the nonamer is 12 base pairs long on both sides of the D segment. As the space separating the two signal sequences in VH DNAs and JH DNAs is 23 +/- 1 base pairs long, the two recombinations required for creation of a complete immunoglobulin VH gene, a VH--D joining and a D--JH joining, follow a 12/23-base pair spacer rule. Allelic exclusion is discussed with respect to D segments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Binding Sites, Antibody / genetics*
  • Chromosome Deletion
  • Genes
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Variable Region / genetics*
  • Mice
  • Myeloma Proteins / genetics
  • Recombination, Genetic


  • Immunoglobulin Heavy Chains
  • Immunoglobulin Variable Region
  • Myeloma Proteins

Associated data

  • GENBANK/J00440
  • GENBANK/J00480
  • GENBANK/J00498
  • GENBANK/V01524