Identification of a mitochondrial phosphoprotein in brain synaptic membrane preparations

J Neurochem. 1981 May;36(5):1861-4. doi: 10.1111/j.1471-4159.1981.tb00442.x.


A 41,000-dalton phosphoprotein in crude synaptosomal membrane fractions is characterized by its unique divalent and monovalent cation regulation. It is identified by two-dimensional gel electrophoresis as the phosphoprotein whose phosphorylation is enhanced by repetitive electrical stimulation of hippocampal brain slices. After sucrose-gradient ultracentrifugation, this phosphoprotein is found in the mitochondrial subfraction. This suggests that the electrically produced changes in the level of phosphorylation of the 41,000-dalton polypeptide are probably effects on cellular energetics rather than on specialized neural membrane function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cerebral Cortex / metabolism*
  • Edetic Acid / pharmacology
  • Humans
  • Mice
  • Mitochondria / metabolism*
  • Molecular Weight
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Potassium / pharmacology
  • Rats
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism*


  • Phosphoproteins
  • Edetic Acid
  • Potassium