Protection of neocortical tissue prisms from freeze-thaw injury by dimethyl sulphoxide

J Neurochem. 1981 Jul;37(1):243-6. doi: 10.1111/j.1471-4159.1981.tb05315.x.


Neocortical tissue prisms prepared from rat and human brain were frozen to -196 degrees C by a two-step freezing procedure and 10% dimethyl sulphoxide as cryoprotectant. Frozen and thawed rat neocortical prisms incorporated glucose into acetylcholine and carbon dioxide at 89% and 86% of control values, respectively, and noradrenaline uptake into frozen and thawed rat prisms was 94% of the control value. Frozen and thawed prisms from three human neocortical specimens showed a similar degree of protection from freeze-thaw injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / biosynthesis
  • Animals
  • Carbon Dioxide / metabolism
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism*
  • Dimethyl Sulfoxide* / pharmacology*
  • Freezing
  • Glucose / metabolism
  • Humans
  • Norepinephrine / metabolism
  • Rats


  • Carbon Dioxide
  • Glucose
  • Acetylcholine
  • Norepinephrine
  • Dimethyl Sulfoxide