Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells

J Immunol. 1981 Sep;127(3):1256-8.

Abstract

The monoclonal antibody OKT-9 recognizes a surface protein of human lymphocytes that consists of a disulfide bonded homodimer of m.w. 200,000 intact and 95,000 reduced. A similar protein is precipitated by transferrin-agarose, but not by agarose alone. Peptide mapping by limited proteolysis shows that the proteins precipitated by OKT-9 antibodies and transferrin-agarose are homologous. It is concluded that OKT-9 antibodies recognize the transferrin receptor. Expression of receptors for transferrin may be useful as a marker for activated or dividing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies*
  • Antibodies, Monoclonal
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Leukemia, Lymphoid / immunology*
  • Mice
  • Molecular Weight
  • Receptors, Immunologic*
  • Sheep
  • Transferrin / metabolism*

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Receptors, Immunologic
  • Transferrin