Abstract
The baculovirus, Autographa california nuclear polyhedrosis virus, induced a new aphidicolin-sensitive, alpha-like, DNA polymerase upon infection of the lepidopteran noctuid, Trichoplusia ni. The new virus-induced DNA polymerase could be separated from the host alpha-like polymerase by phosphocellulose chromatography. The two polymerases differed in their sensitivities to heat inactivation, high salt concentrations, and 0.1 M phosphate buffer.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Aphidicolin
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Buffers
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DNA-Directed DNA Polymerase / biosynthesis*
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DNA-Directed DNA Polymerase / metabolism
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Diterpenes / pharmacology
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Enzyme Induction
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Hot Temperature
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Insect Viruses / enzymology*
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Lepidoptera / microbiology
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Nucleic Acid Synthesis Inhibitors
Substances
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Buffers
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Diterpenes
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Nucleic Acid Synthesis Inhibitors
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Aphidicolin
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DNA-Directed DNA Polymerase