Sensitive solid phase enzyme immunoassay for human IgA, secretory IgA, and secretory component

Clin Chim Acta. 1981 Oct 26;116(2):237-43. doi: 10.1016/0009-8981(81)90028-0.

Abstract

Highly sensitive solid-phase immunoassay systems for human immunoglobulin A (IgA), secretory component (SC), secretory immunoglobulin A (SIgA) were developed by use of antisera against the alpha-chain of IgA and SC, and beta-D-galactosidase from Escherichia coli as label. IgA and SC wee assayed with the respective solid-phase (silicone rubber)-immobilized F(ab')2 antibody fragments and the corresponding antibody Fab'-beta-D-galactosidase complex. More than 1 and 0.4 fmol (or 0.16 and 0.03 ng) of IgA, and SC, respectively, were determined, but the assay system for IgA and that for SC cross-reacted with SIgA about 90% and 2%, respectively. SIgA was specifically determined in an assay using silicone rubber with immobilized (anti-alpha-chain)F(ab')2 fragments and (anti-SC) Fab'-beta-D-galactosidase complex with a minimum detectable sensitivity of 2.5 fmol or 1 ng. IgA and SC values could be corrected by subtracting the amounts of cross-reacting SIgA in the same samples. Small amounts of SIgA, SC, and IgA in saliva, sweat, urine, and feces could be determined by the present method.

MeSH terms

  • Child, Preschool
  • Feces / analysis
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin A / analysis*
  • Immunoglobulin A / urine
  • Immunoglobulin Fab Fragments
  • Immunoglobulin Fragments / analysis*
  • Infant
  • Male
  • Saliva / analysis
  • Secretory Component / analysis*
  • Secretory Component / urine
  • Sweat / analysis
  • beta-Galactosidase

Substances

  • Immunoglobulin A
  • Immunoglobulin Fab Fragments
  • Immunoglobulin Fragments
  • Secretory Component
  • beta-Galactosidase