Intraspecific and Intergeneric Mobilization of Non-Conjugative Resistance Plasmids by a 24.5 Megadalton Conjugative Plasmid of Neisseria Gonorrhoeae

J Gen Microbiol. 1981 Jul;125(1):123-9. doi: 10.1099/00221287-125-1-123.

Abstract

pLE2451, a 24.5 megadalton conjugative plasmid from Neisseria gonorrhoeae, was capable of efficiently mobilizing gonococcal beta-lactamase plasmids between gonococci and from gonococci to Haemophilus influenzae and restriction-deficient Escherichia coli. Donor strains of N. gonorrhoeae carrying pLE2451 were also found to be capable of mobilizing a variety of non-conjugative plasmids originally derived from enteric bacteria or Haemophilus species when such plasmids were resident in E. coli. Nevertheless, pLE2451 was not detected physically in E. coli or H. influenzae transconjugants. This suggests that the plasmid is unstable in these hosts but survives transiently to provide transfer functions for mobilization. The proficiency of pLE2451 in promoting intraspecific and intergeneric mobilization was not paralleled by pUB701, pRI234 and pFR16017, a series of conjugative plasmids derived originally from Haemophilus species. These plasmids were incapable of mobilizing even Haemophilus beta-lactamase plasmids, such as RSF0885, between Haemophilus species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conjugation, Genetic
  • Escherichia coli / genetics
  • F Factor*
  • Haemophilus influenzae / genetics
  • Neisseria gonorrhoeae / enzymology
  • Neisseria gonorrhoeae / genetics*
  • Penicillin Resistance
  • Penicillinase / genetics
  • Plasmids*
  • R Factors*

Substances

  • Penicillinase