Isolation of Alginate-Producing Mutants of Pseudomonas Fluorescens, Pseudomonas Putida and Pseudomonas Mendocina

J Gen Microbiol. 1981 Jul;125(1):217-20. doi: 10.1099/00221287-125-1-217.


Spontaneous alginate-producing (muc) variants were isolated from strains of Pseudomonas fluorescens, P. putida and P. mendocina at a frequency of 1 in 10(8) by selecting for carbenicillin resistance. The infrared spectrum of the bacterial exopolysaccharide was typical of an acetylated alginate similar to that previously described in Azotobacter vinelandii and in mucoid variants of P. aeruginosa. Mucoid variants were not isolated from P. stutzeri, P. pseudoalcaligenes, P. testosteroni, P. diminuta, P. acidovorans, P. cepacia or P. maltophilia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / biosynthesis*
  • Glucuronic Acid
  • Hexuronic Acids
  • Mutation
  • Pseudomonas / genetics*
  • Pseudomonas / isolation & purification
  • Pseudomonas / metabolism
  • Pseudomonas fluorescens / genetics
  • Pseudomonas fluorescens / isolation & purification
  • Pseudomonas fluorescens / metabolism


  • Alginates
  • Hexuronic Acids
  • Glucuronic Acid