Construction of a functional human suppressor tRNA gene: an approach to gene therapy for beta-thalassaemia

Nature. 1982 Apr 8;296(5857):537-40. doi: 10.1038/296537a0.


A human tRNALys gene was converted to an amber suppressor by site-specific mutagenesis of the anticodon. The mutated tRNALys gene directed synthesis of a tRNA that suppressed the UAG amber nonsense mutation in beta O thalassemia mRNA. Such genes may be used to detect other nonsense mutations in mammalian cells and may provide an approach to gene therapy for beta O thalassaemia due to nonsense mutations.

MeSH terms

  • Anticodon
  • Genes
  • Humans
  • Lysine
  • Mutation
  • Peptide Chain Termination, Translational
  • RNA, Transfer / genetics*
  • Suppression, Genetic*
  • Thalassemia / genetics
  • Thalassemia / therapy*
  • Transcription, Genetic


  • Anticodon
  • RNA, Transfer
  • Lysine