Exchange of colicin receptor capacity between strains of Escherichia coli sensitive or resistant to colicin K-K235

Arch Microbiol. 1982 May;131(3):229-34. doi: 10.1007/BF00405884.


Phage and colicin-resistant mutants were derived from Escherichia coli K-12P678. Two classes of phage T6 and colicin K-resistant mutants (genotype tsx) were isolated. Tsx-2 mutants, which demonstrated mucoid growth and increased sensitivities to many antibiotics, became sensitive to colicin K when pretreated with ethylenediaminetetraacetate (EDTA), whereas Tsx-1 mutants did not. Reassociation of EDTA-released material partially restored resistance to colicin K for Tsx-2 mutants. When EDTA-released material from strain P678 was associated with either class of K-resistant mutant, an increase in colicin K sensitivity resulted. Observations suggest that colicin K can act on its target site once it penetrates the cell surface. In addition, results suggest that functional colicin K receptors can be transferred from sensitive to resistant strains, thus conferring colicin sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / metabolism
  • Colicins / metabolism*
  • Colicins / pharmacology
  • Coliphages / genetics
  • Drug Resistance, Microbial
  • Edetic Acid / pharmacology
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Escherichia coli Proteins*
  • Kinetics
  • Membrane Proteins / isolation & purification
  • Receptors, Cell Surface*
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism


  • Colicins
  • Escherichia coli Proteins
  • Membrane Proteins
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • colicin receptor, E coli
  • Edetic Acid