Molecular characterization of ribosomal genes on the Ybb- chromosome of Drosophila melanogaster

Genetics. 1982 Sep;102(1):91-9. doi: 10.1093/genetics/102.1.91.


The question of whether the Ybb- chromosome contains ribosomal genes has been examined by using Southern blot analysis and comparing rDNA hybridization patterns for X/X and X/Ybb- DNA. The results demonstrate that the Ybb- chromosome contains sequences that hybridize to an rDNA probe under stringent conditions. Differential hybridization of some of these sequences with DNAs corresponding to different regions of a complete ribosomal gene repeat provides evidence that some of the genes on the Ybb- chromosome are type 2 repeats. Because data obtained by other workers suggest that type 2 repeats are transcribed only to low levels, these repeats may be classed as "nonfunctional". A further finding is that the ribosomal genes on the Ybb- chromosome do not undergo multiple rounds of DNA replication during polytenization of X/Ybb- cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromosome Mapping
  • DNA / analysis
  • Drosophila melanogaster / genetics*
  • Genes*
  • Male
  • Nucleic Acid Hybridization
  • RNA, Ribosomal / genetics*
  • Sex Chromosomes / ultrastructure*
  • Y Chromosome / ultrastructure*


  • RNA, Ribosomal
  • DNA