The preparation and partial characterization of N-terminal and C-terminal iron-binding fragments from rabbit serum transferrin

Biochem J. 1982 Sep 1;205(3):611-7. doi: 10.1042/bj2050611.

Abstract

Two iron-binding fragments of Mr 36 000 and 33 000 corresponding to the N-terminal domain of rabbit serum transferrin were prepared. One iron-binding fragment of Mr 39 000 corresponding to the C-terminal domain was prepared. The N-terminal amino acid sequence of rabbit serum transferrin is: Val-Thr-Glu-Lys-Thr-Val-Asn-Trp-?-Ala-Val-Ser. One glycan unit is presented in rabbit serum transferrin and it is located in the C-terminal domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Electrophoresis, Polyacrylamide Gel
  • Hexoses
  • Immunodiffusion
  • Iron / metabolism*
  • Peptide Fragments / immunology
  • Peptide Fragments / isolation & purification
  • Peptide Fragments / metabolism*
  • Rabbits
  • Transferrin / blood*
  • Transferrin / immunology

Substances

  • Hexoses
  • Peptide Fragments
  • Transferrin
  • Iron