Eukaryotic RNA polymerase II binds to nucleosome cores from transcribed genes

Nature. 1983 Feb 10;301(5900):482-8. doi: 10.1038/301482a0.

Abstract

Purified RNA polymerase II from calf thymus can bind to about 15% of the nucleosome core particles prepared from mouse myeloma cells, forming a discrete complex having a sedimentation coefficient of 18S. These bound nucleosome cores are heavily enriched in transcribed DNA sequences, are deficient in histones H2A and H2B, and undergo a reversible change in structure on RNA polymerase II binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • DNA-Directed RNA Polymerases / genetics*
  • Genes
  • Histones / genetics
  • Mice
  • Nucleosomes / physiology*
  • Nucleosomes / ultrastructure
  • Protein Binding
  • RNA Polymerase II / genetics*
  • Transcription, Genetic*

Substances

  • Histones
  • Nucleosomes
  • RNA Polymerase II
  • DNA-Directed RNA Polymerases