Theoretical analysis of fluorescence photobleaching recovery experiments

Biophys J. 1983 Jan;41(1):95-7. doi: 10.1016/S0006-3495(83)84410-5.


We derive an exact closed formula for the fluorescence recovery curve measured in fluorescence photobleaching recovery experiments employing uniform circular laser beams. In contrast to the expression used currently, this result is very simple and free of mathematical drawbacks, thus facilitating the quantitative analysis of experimental data.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Mathematics
  • Membranes / analysis*
  • Models, Biological
  • Photochemistry*
  • Spectrometry, Fluorescence / methods*