Clinical applications of fluorometry

Doc Ophthalmol. 1983 Feb 28;55(1-2):37-40. doi: 10.1007/BF00140458.


An instrument has been developed that measures in ten seconds the level of fluorescein along a scanning line from the retina to the cornea. With spatial resolving power of 1.1 mm in the eye, concentrations of 1 X 10(-8) gr/ml fluorescein and higher are detectable. In this way an indication of the integrity of the blood-ocular barriers is achieved. In uveitis the dysfunction is mainly localised at the blood-aqueous barrier while in diabetic retinopathy the blood-retinal barrier is predominantly affected. Sources of error are discussed.

MeSH terms

  • Adult
  • Choroid Neoplasms / diagnosis
  • Diabetic Retinopathy / diagnosis*
  • Eye / blood supply
  • Eye Diseases / diagnosis*
  • Female
  • Fluorometry / instrumentation*
  • Humans
  • Male
  • Melanoma / diagnosis
  • Middle Aged
  • Uveitis / diagnosis*