Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis

Exp Cell Res. 1983 Mar;144(1):73-82. doi: 10.1016/0014-4827(83)90443-3.

Abstract

A fluorescent phallotoxin with high photostability, tetramethylrhodaminyl-phalloidin (Rh-phalloidin), has been prepared. The affinity of this compound to rabbit muscle actin has been determined to be about 6 times lower than that of phalloidin. In freshly isolated hepatocytes the internalized fluorescent toxin stains the cellular actin. In contrary, there is no actin staining visible in cultured hepatocytes. Short-term cultured hepatocytes (5 h of culturing) incorporate the toxin by endocytosis; it is kept sealed in the endocytotic vesicles, which are usually found accumulated at the sites where cells touch after reaggregation.

MeSH terms

  • Actins / metabolism
  • Animals
  • Cells, Cultured
  • Endocytosis*
  • Liver / cytology*
  • Liver / metabolism
  • Male
  • Oligopeptides / metabolism*
  • Phalloidine / metabolism*
  • Phalloidine / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Rhodamines

Substances

  • Actins
  • Oligopeptides
  • Rhodamines
  • Phalloidine
  • tetramethylrhodamine