Dye interactions. A basis for specific detection and histochemistry of polysaccharides

J Histochem Cytochem. 1983 Jun;31(6):823-6. doi: 10.1177/31.6.6841974.

Abstract

Specific histochemical staining can be related to specific interactions of dyes and polysaccharides in solution. Specificity evidently depends upon polysaccharide conformation and the need for a close "fit" between polysaccharide and ligand. As a consequence, microscopic identification of cereal beta-(1 leads to 4)(1 leads to 3)-D-glucan and beta-(1 leads to 3)-D-glucan is possible, and is demonstrated.

MeSH terms

  • Benzenesulfonates
  • Carbohydrate Conformation
  • Congo Red
  • Glucans / analysis*
  • Histocytochemistry
  • Methods

Substances

  • Benzenesulfonates
  • Glucans
  • cellu-brite
  • Congo Red