Cell kinetics and sister-chromatid-exchange frequency in human lymphocytes

Mutat Res. 1983 May;120(2-3):193-9. doi: 10.1016/0165-7992(83)90163-x.


Repeated cultures from 5 healthy donors were set up in 3 tissue-culture media: TC109, TC199 and TC1640. For each donor, 3 blood samples were taken at 1-week intervals, making a total of 5 x 3 x 3 = 45 cultures. Our results show a large variability in the individual SCE base-line frequency. This variability can be partly attributed to the different tissue-culture media or to the donors themselves. Our results, however, stress the importance of the proliferating rate of the culture in determining the SCE frequency.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Cells, Cultured
  • Chromatids / ultrastructure*
  • Crossing Over, Genetic*
  • Culture Media
  • Female
  • Humans
  • Kinetics
  • Lymphocytes / ultrastructure*
  • Male
  • Mitotic Index
  • Sister Chromatid Exchange*
  • Time Factors


  • Culture Media