51Cr release cytotoxicity radioimmunoassay to detect immune cytotoxic reactions to rat Schwann cells in vitro

Neurosci Lett. 1983 Mar 14;35(3):321-6. doi: 10.1016/0304-3940(83)90338-5.


Monolayers of rat Schwann cells incubated with 51Cr were used as targets for a cytotoxicity assay employing rabbit antiserum to galactocerebroside (R anti-GalC). Specific 51Cr release was demonstrated which was heat sensitive and complement-dependent. The assay was readily performed, reproducible and quantitative, and should prove useful in assessing anti-Schwann cell cytotoxic activity of experimental and human serum.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chromium*
  • Cytotoxicity Tests, Immunologic / methods*
  • In Vitro Techniques
  • Radioimmunoassay / methods*
  • Rats
  • Rats, Inbred Strains
  • Schwann Cells / immunology*


  • Chromium