The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase

Arch Biochem Biophys. 1983 Jun;223(2):639-48. doi: 10.1016/0003-9861(83)90628-8.


Epoxyeicosatrienoic acids, formed during the cytochrome P-450-catalyzed oxidation of arachidonic acid, react with a liver cytosolic epoxide hydrolase to form vicinal diols of eicosatrienoic acid. The role of this cytosolic enzyme, rather than a microsomal bound type, explains previous results illustrating the ability to accumulate epoxides during the in vitro aerobic steady state of oxidative metabolism of arachidonic acid by liver microsomes. The inability of the 5,6-epoxyeicosatrienoic acid to serve as a suitable substrate for this enzyme is discussed in light of recent studies concerning possible unique physiological functions for this metabolite.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachidonic Acids / chemical synthesis
  • Arachidonic Acids / metabolism*
  • Cell-Free System
  • Cytosol / enzymology
  • Epoxide Hydrolases / metabolism*
  • Epoxy Compounds
  • Kinetics
  • Liver / enzymology*
  • Male
  • Microsomes, Liver / metabolism
  • Rats


  • Arachidonic Acids
  • Epoxy Compounds
  • Epoxide Hydrolases