Structural organization of filamentous proteins in postsynaptic density

Biochemistry. 1983 May 10;22(10):2446-53. doi: 10.1021/bi00279a022.


Actin is one of the major protein constituents of the postsynaptic density (PSD), a characteristic structural entity subjacent to the postsynaptic membrane in excitatory synapses of the vertebrate central nervous system. In isolated purified PSD preparations, it is present to the extent of 29 +/- 2 micrograms/mg of total protein, 90% of which is in the filamentous (F-actin) form. Iodination by a discriminatory labeling technique demonstrates that actin is located on the surface of the PSD from which it can be stripped by treatment with a mixture of strong anionic detergents, leaving behind an insoluble core held together by disulfide bridges, consisting in part of tubulin and "PSD protein".

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / isolation & purification
  • Actins / physiology*
  • Animals
  • Cerebral Cortex / physiology*
  • Male
  • Molecular Weight
  • Peptide Fragments / analysis
  • Rats
  • Rats, Inbred Strains
  • Sulfhydryl Compounds / analysis
  • Synapses / physiology*


  • Actins
  • Peptide Fragments
  • Sulfhydryl Compounds