Kinetic evidence for two separate trans-2-enoyl CoA reductases in rat hepatic microsomes: NADPH-specific short chain- and NAD(P)H-dependent long chain-reductase

Biochem Biophys Res Commun. 1983 Jun 15;113(2):659-65. doi: 10.1016/0006-291x(83)91777-1.

Abstract

The rat hepatic microsomal conversion of crotonyl- and hexenoyl CoA to butyrate and hexanoate was supported only by NADPH, while both NADH and NADPH were effective cofactors in the conversion of trans-2-hexadecenoyl CoA to palmitate. Experiments using mixtures of long- and short-chain enoyl-CoA substrates and competition experiments support the conclusion that microsomes contain 2 distinct enoyl CoA reductases, (1) a long chain enoyl CoA reductase capable of accepting reducing equivalents from either NADH or NADPH, and (2) a NADPH-specific short chain enoyl CoA reductase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chemical Phenomena
  • Chemistry
  • Fatty Acid Desaturases / classification
  • Fatty Acid Desaturases / isolation & purification*
  • In Vitro Techniques
  • Kinetics
  • Male
  • Microsomes, Liver / enzymology*
  • NADP / metabolism*
  • Rats
  • Rats, Inbred Strains

Substances

  • NADP
  • Fatty Acid Desaturases