A treadmill for equine locomotion analysis is described and its potential considered for locomotive research and clinical investigation. The treadmill comprised an endless belt driven by a hydraulic motor at various speeds up to 14 m/sec and the direction of belt movement was reversible. The carrying side of the belt ran over a steel-concrete table which acted as a flat support. The belt itself consisted of a steel base on to which was glued a rubber belt and the surface was covered with a layer of coir matting which permitted some forward sliding of the landing hooves simulating the conditions on an ordinary track. The treadway could be inclined up to 10 degree in the longitudinal and 7.5 degrees in the transverse direction. To minimise the risk of injuries a special emergency stop system was installed. Horses adapted well to exercising on the treadmill, even without any previous experience. A good correlation was found between the gait repetitiveness on the racetrack and that displayed on the treadmill. One horse recorded both on the racetrack and on the treadmill showed significantly shorter strides on the treadmill but this did not invalidate the treadmill as a useful research tool in the study of equine locomotion.