Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes

J Immunol Methods. 1983 Aug 12;62(1):31-7. doi: 10.1016/0022-1759(83)90107-2.

Abstract

A simple and efficient technique to purify human peripheral blood monocytes is described. This technique is based on the fact that monocytes have high affinity for fibronectin immobilized on a gelatin coated surface. Cell preparations obtained by the method described are more than 90% monocytes. The phenotype of these adherent cells was characterized with monoclonal antibodies.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Line
  • Cell Separation / methods
  • Cricetinae
  • Gelatin
  • Humans
  • Kidney
  • Monocytes / cytology*
  • Monocytes / immunology
  • Phenotype
  • Plasma

Substances

  • Gelatin