The intracellular Na activity, aNai, and the intracellular Ca activity, aCai, were measured with double-barrelled neutral carrier Na+- and Ca2+-sensitive microelectrodes in Retzius neurones in the central nervous system of the leech Hirudo medicinalis. The aNai was measured to be 8.0 mM (corrected for Ca interference), which corresponds to a cytoplasmic Na+ concentration of 10.7 mM, assuming a Na activity coefficient of 0.75. The calculated Na+ equilibrium potential was 59 mV, giving a total Na+ electrochemical gradient of approximately 102 mV. The aCai was found to range between 1 and 5 X 10(-7) M, from which a Ca2+ equilibrium potential near + 120 mV was estimated. When the Na+-K+ pump was inhibited by lowering the external K+ concentration or by adding the glycoside ouabain (5 X 10(-4) M), the aNai reversibly increased severalfold. When aNai increased to high levels following complete pump inhibition, the aCai increased above 10(-6) M, and the membrane input resistance decreased. After removal of ouabain, aNai, aCai and the membrane resistance recovered within 30 min after a delay of 20-40 min. Our results suggest that a large increase of aNai produces a rise in aCai, possibly by means of a Na+-Ca2+ exchange across the cell membrane. The elevation of the aCai may be responsible for the decrease in membrane resistance, and may also be related to the uncoupling of the paired Retzius neurones observed in the presence of Na+-K+ pump inhibitors.