Evaluation of factors affecting mortality rate after sepsis in a murine cecal ligation and puncture model

Surgery. 1983 Aug;94(2):331-5.


A murine model was used to test the effects of various therapeutic modalities on the rate of death following intra-abdominal sepsis as produced by cecal ligation and puncture (CLP). There were no deaths among sham-operated control mice after ether anesthesia, whereas CLP produced a mortality rate of 100% by 24 hours. When CLP was followed at 16 hours by excision of the cecum and saline peritoneal lavage (CLPE), the mortality rate was 20% at 24 hours and 60% at 72 hours. The therapeutic modalities consisted of gentamicin (1.5 mg/kg) alone or in combination with methylprednisolone (50 mg/kg) or tuftsin (1 mg/kg) administered before CLP and at 16 and 24 hours after CLP. Separate groups of animals also received only methylprednisolone or tuftsin, a tetrapeptide produced by the spleen. Compared with the mortality rate in the CLPE group, mortality at 24 and 72 hours was decreased for gentamicin alone (0% and 10%, respectively), tuftsin alone (10%, 40%), or the two in combination (0%, 20%). As compared with CLPE, methylprednisolone led to increased mortality rates at 24 and 72 hours (70%, 80%). The data (significant at P less than 0.01, X2 analysis) suggest that gentamicin and tuftsin may improve the rate of early survival after intra-abdominal sepsis in this Model. Steroids do not seem to be beneficial and may, in fact, be harmful.

Publication types

  • Comparative Study

MeSH terms

  • Anesthesia / mortality
  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Bacterial Infections / drug therapy
  • Bacterial Infections / mortality*
  • Cecum / surgery*
  • Disease Models, Animal*
  • Drug Therapy, Combination
  • Ether
  • Evaluation Studies as Topic
  • Female
  • Ligation
  • Male
  • Mice
  • Mice, Inbred DBA
  • Premedication*
  • Punctures
  • Therapeutic Irrigation
  • Time Factors


  • Anti-Bacterial Agents
  • Ether