Partial characterization of a cubic Bacillus phage

Can J Microbiol. 1978 Aug;24(8):986-93. doi: 10.1139/m78-162.

Abstract

Partial characterization of a cubic Bacillus phage. Can. J. Microbiol. 24: 986--993. Phage Bam35 is an icosahedron of about 63 nm in diameter. It has a double capsid with spikes at the vertices, and a tail which seems to appear upon nucleic acid ejection. The phage contains DNA and, probably, lipids which seem to be located in the inner coat. The phage is Bacillus-specific, UV- and lipase-resistant, and sensitive to heat, chloroform, and ether. The latent period is 50 min and the burst size is 39. Page Bam35 belongs to a new virus group which includes a phage of B. anthracis and four phages of gram-negative bacteria harboring drug-resistance plasmids.

MeSH terms

  • Bacillus thuringiensis
  • Bacteriophage Typing
  • Bacteriophages / classification
  • Bacteriophages / isolation & purification
  • Bacteriophages / physiology*
  • Chloroform / pharmacology
  • Culture Techniques
  • Drug Resistance, Microbial
  • Ether / pharmacology
  • Hot Temperature
  • Lipase / pharmacology
  • Microbial Sensitivity Tests
  • Ultraviolet Rays

Substances

  • Ether
  • Chloroform
  • Lipase