Binding of monoclonal antibodies to the nuclear estrogen receptor in intact nuclei

Biochem Biophys Res Commun. 1983 Jul 18;114(1):107-12. doi: 10.1016/0006-291x(83)91600-5.

Abstract

A monoclonal antibody to calf uterus cytoplasmic estrogen receptor shows a specifically displaceable and saturable binding to intact nuclei of mouse uterus after estradiol stimulation. The binding is complete after 3 hr at 0 degree C. The binding of the antibody correlates with the exchangeable estradiol binding activity of the nuclei over a 4-hr time course following in vivo injection of 17 beta-estradiol.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antigen-Antibody Complex
  • Castration
  • Cattle
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism*
  • Cytosol / metabolism
  • Estradiol / metabolism*
  • Estradiol / pharmacology
  • Female
  • Kinetics
  • Mice
  • Receptors, Estradiol
  • Receptors, Estrogen / immunology
  • Receptors, Estrogen / metabolism*
  • Uterus / metabolism*

Substances

  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Receptors, Estradiol
  • Receptors, Estrogen
  • Estradiol