A method for the purification of placental protein 5 (PP5) from placental extracts

Clin Chim Acta. 1983 Jul 15;131(3):289-94. doi: 10.1016/0009-8981(83)90098-0.

Abstract

We describe a method for the preparation of purified PP5 suitable for use as a tracer ligand in RIA. The method is based on the observation that PP5 will bind to heparin and employs a heparin-Sepharose column as a key step in the procedure.

MeSH terms

  • Chromatography / methods
  • Electrophoresis, Polyacrylamide Gel / methods
  • Female
  • Glycoproteins*
  • Humans
  • Placenta / analysis*
  • Pregnancy
  • Pregnancy Proteins / isolation & purification*
  • Radioimmunoassay / methods
  • Tissue Extracts / analysis*

Substances

  • Glycoproteins
  • Pregnancy Proteins
  • Tissue Extracts
  • tissue-factor-pathway inhibitor 2