Abstract
We describe a method for the preparation of purified PP5 suitable for use as a tracer ligand in RIA. The method is based on the observation that PP5 will bind to heparin and employs a heparin-Sepharose column as a key step in the procedure.
MeSH terms
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Chromatography / methods
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Electrophoresis, Polyacrylamide Gel / methods
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Female
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Glycoproteins*
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Humans
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Placenta / analysis*
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Pregnancy
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Pregnancy Proteins / isolation & purification*
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Radioimmunoassay / methods
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Tissue Extracts / analysis*
Substances
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Glycoproteins
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Pregnancy Proteins
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Tissue Extracts
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tissue-factor-pathway inhibitor 2