A high performance liquid chromatographic method of analysis of 4'-O-tetrahydropyranyladriamycin and their metabolites in biological samples

J Antibiot (Tokyo). 1983 Jul;36(7):880-6. doi: 10.7164/antibiotics.36.880.


A method for measuring 4'-O-tetrahydropyranyladriamycin (THP) and its metabolites in biological samples are described. By reversed-phase high performance liquid chromatography using fluorescence detection, THP and its metabolites were all separated on a single chromatogram within 18 minutes. A linear calibration curve was obtained up to 2,000 ng/ml of THP in plasma. The recovery of THP in the analysis was more than 95% above 5 ng/ml and 87.1% even at 1.25 ng/ml. Thus the lower limit was 1.25 ng/ml in biological samples. Blood levels and urinary excretion in mice and dogs were satisfactory measured by this analytical method.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid / methods
  • Dogs
  • Doxorubicin / analogs & derivatives*
  • Doxorubicin / blood
  • Doxorubicin / metabolism
  • Doxorubicin / urine
  • Indicators and Reagents
  • Kinetics
  • Male
  • Mice
  • Mice, Inbred Strains


  • Indicators and Reagents
  • Doxorubicin
  • pirarubicin