X-chromosome instability in pluripotential stem cell lines derived from parthenogenetic embryos

J Embryol Exp Morphol. 1983 Apr;74:297-309.

Abstract

The karotype of six pluripotential stem cell lines derived from haploid and two additional lines derived from diploid parthenogenetic embryos is described. All these lines are diploid and possess a normal autosomal complement. The stage at which diploidization of the haploid cells occurs is not yet known. The XX-chromosome complement in these lines is unstable, although in two haploid-derived lines and one diploid-derived line many normal XX-bearing cells are found in early cultures. All of the lines so far examined either become XO (rarely), or a single X-chromosome shows a deletion in the distal region. The extent of this deletion varies between lines, but the position of the breakpoint appears to be constant for a given line. We suggest that these cytogenetic findings raise the possibility that a single deletion event occurring at an early stage during the isolation of these lines may confer a selective advantage to those cells carrying the deleted X chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / ultrastructure*
  • Cell Line
  • Cells, Cultured
  • Chromosome Deletion
  • Diploidy
  • Female
  • Haploidy
  • Karyotyping
  • Mice
  • Mice, Inbred Strains
  • Parthenogenesis
  • Sex Chromosomes / physiology*
  • X Chromosome / physiology*