Intrathecal morphine in mice: a new technique

Eur J Pharmacol. 1980 Oct 17;67(2-3):313-6. doi: 10.1016/0014-2999(80)90515-4.


A simple, rapid technique for intrathecal injections by lumbar puncture in unanesthetized mice is described. Intrathecal [3H]morphine base was not found in significant quantities in either the midbrain or forebrain. Submicrogram quantities of morphine sulfate induced Straub tail response and tail-flick analgesia. These effects were dose related and antagonized by subcutaneous naloxone.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analgesia
  • Animals
  • Dose-Response Relationship, Drug
  • Injections, Spinal
  • Male
  • Mice
  • Morphine / administration & dosage*
  • Morphine / pharmacology
  • Spinal Cord / drug effects*


  • Morphine