Amino acid sequence of the tryptic peptide containing the catalytic-site thiol group of bovine liver uridine diphosphate glucose dehydrogenase

Biochem J. 1981 Dec 1;199(3):599-602. doi: 10.1042/bj1990599.


The catalytic-site thiol groups of UDP-glucose dehydrogenase from bovine liver were carboxymethylated with iodo[2-14C]acetate or with iodoacetamidofluorescein. After the residual thiol groups were carboxymethylated with iodoacetate, the proteins were digested with trypsin. The 14C-labelled peptide from the carboxymethylated enzyme was purified to homogeneity by successive thick-layer chromatography on silica gel, paper electrophoresis and chromatography, and column chromatography on Bio-Gel P-6. Homogeneous fluoresceincarboxamidomethylated peptide was prepared from a tryptic digest of fluoresceincarboxamidomethylated enzyme by specific adsorption--desorption from Sephadex G-25. The sequences of either peptide determined by the manual Edman dansyl procedure is: Ala-Ser-Val-Gly-Phe-Gly-Gly-Ser-Cys-Phe-Glx-Glx-Gly-Lys.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Carbohydrate Dehydrogenases / metabolism*
  • Catalysis
  • Cattle
  • Fluoresceins
  • Fluorescent Dyes
  • Liver / enzymology*
  • Peptide Fragments / isolation & purification*
  • Sulfhydryl Compounds / metabolism
  • Trypsin / metabolism
  • Uridine Diphosphate Glucose Dehydrogenase / metabolism*


  • Fluoresceins
  • Fluorescent Dyes
  • Peptide Fragments
  • Sulfhydryl Compounds
  • 5-iodoacetamidofluorescein
  • Carbohydrate Dehydrogenases
  • Uridine Diphosphate Glucose Dehydrogenase
  • Trypsin