Pancreatic spasmolytic polypeptide (PSP): I. Preparation and initial chemical characterization of a new polypeptide from porcine pancreas

Regul Pept. 1982 Mar;3(3-4):207-19. doi: 10.1016/0167-0115(82)90126-4.


A novel polypeptide, named Pancreatic Spasmolytic Polypeptide (PSP), was discovered in a side-fraction from the purification of porcine insulin. PSP was prepared by two different purification methods based on combinations of precipitations, anion-exchange and cation-exchange chromatography. The highest yield obtained, 52 mg PSP/kg pancreas, indicates that the content of PSP in porcine pancreas is about half the content of insulin. Both preparations appeared to be very pure as judged by basic disc electrophoresis, isoelectric focusing, analytical gel filtration and radioimmunoassays for various polypeptides known to be present in pancreas. The PSP molecule contains 106 amino acids (MW about 11 700). PSP is an acidic (pI 4.4), non-glycosylated protein without free N-terminal amino groups, and with high contents of proline and cystine. The high content of S-S bridges (7 per molecule), an unexpected low apparent MW determined by gel filtration, and a remarkable resistance towards treatment with trypsin and chymotrypsin, point to a compact structure of the PSP molecule.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Chromatography, Ion Exchange
  • Electrophoresis, Disc
  • Intercellular Signaling Peptides and Proteins
  • Isoelectric Focusing
  • Molecular Weight
  • Mucins*
  • Muscle Proteins*
  • Neuropeptides*
  • Pancreas / analysis*
  • Parasympatholytics / isolation & purification*
  • Parasympatholytics / metabolism
  • Peptides / isolation & purification*
  • Peptides / metabolism
  • Swine
  • Trefoil Factor-2
  • Trefoil Factor-3


  • Amino Acids
  • Intercellular Signaling Peptides and Proteins
  • Mucins
  • Muscle Proteins
  • Neuropeptides
  • Parasympatholytics
  • Peptides
  • TFF3 protein, rat
  • Tff2 protein, rat
  • Trefoil Factor-2
  • Trefoil Factor-3
  • pancreatic spasmolytic polypeptide