Pancreatic spasmolytic polypeptide (PSP): II. Radioimmunological determination of PSP in porcine tissues, plasma and pancreatic juice

Regul Pept. 1982 Mar;3(3-4):221-30. doi: 10.1016/0167-0115(82)90127-6.


A radioimmunoassay (RIA) for the determination of porcine Pancreatic Spasmolytic Polypeptide (PSP) has been developed. The antisera raised in rabbits were sensitive to 5 pgequiv. of PSP in a volume of 100 microliter. Immunoreactive PSP (IR-PSP) has been determined in extracts from 22 porcine organs. Pancreas was found to be the only organ containing substantial amounts of IR-PSP (0.1 mgequiv. IR-PSP/g wet weight). The fasting porcine plasma level of IR-PSP was about 10 ngequiv./ml, corresponding to 850 pM. The concentration of IR-PSP in porcine pancreatic juice varied from 0.2 mugequiv./ml in the fasting state to 46-116 mugequiv./ml after stimulation with pancreozymin or secretin. A linear correlation was found between the exocrine secretion of IR-PSP and total protein.

MeSH terms

  • Animals
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Intercellular Signaling Peptides and Proteins
  • Mucins*
  • Muscle Proteins*
  • Neuropeptides*
  • Pancreas / analysis*
  • Pancreatic Juice / analysis*
  • Parasympatholytics / analysis*
  • Parasympatholytics / blood
  • Peptides / analysis*
  • Peptides / blood
  • Rabbits / immunology
  • Radioimmunoassay / methods
  • Swine
  • Tissue Distribution
  • Trefoil Factor-2
  • Trefoil Factor-3


  • Intercellular Signaling Peptides and Proteins
  • Mucins
  • Muscle Proteins
  • Neuropeptides
  • Parasympatholytics
  • Peptides
  • TFF3 protein, rat
  • Tff2 protein, rat
  • Trefoil Factor-2
  • Trefoil Factor-3
  • pancreatic spasmolytic polypeptide