Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa

J Infect Dis. 1980 Oct;142(4):547-55. doi: 10.1093/infdis/142.4.547.

Abstract

The data presented indicate that in experimental infections of the mouse cornea, toxin A of Pseudomonas aeruginosa contributes to the organism's pathogenicity, whereas active elastase may not be required. After traumatization, corneas were infected with wild-type parental toxin A-producing strains, two toxin A-deficient mutants (Tox-), or an elastase mutant. The infections produced by both Tox- mutants were less severe than infections produced by their parental strains. Furthermore, the Tox- mutants were not able to persist in the eyes as long as their parental strains. Addition of subdamaging doses of exogenous toxin A to eyes infected with the Tox- mutant PA103-29 significantly increased is virulence. The course of infection and the resulting corneal damage produced by the elastase mutant were indistinguishable from those of its parental strain.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Corneal Diseases / etiology*
  • Female
  • Mice
  • Mutation
  • Pancreatic Elastase / genetics*
  • Pseudomonas Infections / etiology*
  • Toxins, Biological*
  • Virulence

Substances

  • Toxins, Biological
  • Pancreatic Elastase