Effect of low dose ionizing radiation on the murine pericryptal fibroblast sheath: radiation damage in a mesenchymal system in vivo

Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Feb;39(2):175-83. doi: 10.1080/09553008114550191.


The effect of low dose ionizing radiation from a variety of sources on the murine small intestinal and colonic pericryptal fibroblast sheath (PCFS) has been investigated. Ultrastructural and light microscopic changes seen within 3 hours after radiation resembles the mode of cell death known as apoptosis. The apoptotic index (AI) was shown to rise steadily for 4 hours after irradiation, and then to return to control levels by 10 hours. 137Cs gamma-irradiation over a range of doses from 0-803 cGy caused an increase in AI from control values of 1-2 per cent to a plateau level of approximately 8.5 per cent for doses above 40 cGy. Investigation of the response of the PCFS to doses of 137Cs and 60Co gamma-irradiation and X-irradiation below 40 cGy demonstrated a steady increase with dose in AI. The survival curve for the radiosensitive subpopulation susceptible to death through apoptosis as a consequence of exposure to non-acute doses of radiation has a D0 of 10.6 cGy and an extrapolation number of 2.3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival / radiation effects
  • Cesium Radioisotopes
  • Cobalt Radioisotopes
  • Colon / radiation effects*
  • Dose-Response Relationship, Radiation
  • Female
  • Fibroblasts / radiation effects
  • Gamma Rays
  • Intestine, Small / radiation effects*
  • Male
  • Mice
  • Mice, Inbred DBA
  • X-Rays


  • Cesium Radioisotopes
  • Cobalt Radioisotopes