Binding of Peanut Lectin to Thymic Cortex and Germinal Centres of Lymphoid Tissue

Immunology. 1981 Apr;42(4):583-91.

Abstract

The binding pattern of horseradish peroxidase conjugated peanut lectin (HRP-PNL) on frozen sections of lymphoid tissue from man, mouse, rat, hamster, guinea-pig, rabbit, sheep and chicken has been investigated. Binding of PNL was found to be highly species dependent; man, mouse and sheep showed strong binding to lymphocytes in thymic cortex and germinal centres; lymphoid tissue from hamster, guinea-pig and rabbit did not stain with HRP-PNL and rat showed only lightly positive cells in thymic cortex and germinal centres; all lymphoid tissue from chicken, except the bursal cortex, bound PNL. Neuraminidase treatment of tissues which did not bind PNL resulted in strongly PNL-positive cells. Double binding studies on murine Peyer's patches with fluorescein isothiocyanate conjugated PNL (FITC-PNL) and tetramethylrhodamine isothiocyanate (TRITC)-anti-Thy-1.2 or anti-immunoglobulin reagents revealed 3%-10% of PNL positive cells to be Thy-1.2 positive and 70%-80% to bear surface immunoglobulin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination
  • Animals
  • Arachis / immunology
  • B-Lymphocytes / drug effects
  • Chickens
  • Cricetinae
  • Guinea Pigs
  • Humans
  • Lectins / immunology*
  • Lymphoid Tissue / immunology*
  • Male
  • Mice
  • Neuraminidase / pharmacology
  • Plant Lectins
  • Rabbits
  • Rats
  • Receptors, Antigen, B-Cell / analysis
  • Sheep
  • Thymus Gland / immunology*

Substances

  • Lectins
  • Plant Lectins
  • Receptors, Antigen, B-Cell
  • Neuraminidase