Antigens displayed on murine B lymphocyte precursors

J Immunol. 1981 Dec;127(6):2262-8.


The surface antigen phenotype of the immediate precursors of clonable B lymphocytes was investigated with conventional alloantisera and monoclonal antibodies directed by B lineage antigens. Ia was demonstrable on B cells, but not their immediate precursors in adult marrow. Adult, but not fetal, B cell precursors were susceptible to lysis with anti-Lyb-2 or anti-Qa. A panel of monoclonal rat antibodies was prepared and placed into categories on the basis of recognition patterns obtained with established cell lines. Of 2 groups that are described here, 1 (typified by antibodies from clone 14.8) detect an antigen that is preferentially expressed on B cells and their precursors, a proportion of antibody-secreting cells, and a subpopulation of peripheral T lymphocytes. Cells that did not display demonstrable amounts of antigen include brain, granulocytes, macrophages, mastocytoma cells, and erythroleukemia cells. A 2nd category of antibodies revealed an antigen that was more widely distributed on hemopoietic cells. Cells capable of quickly maturing into functional, colony-forming B lymphocytes in culture or after transfer to irradiated recipients specifically adhered to 14.8 antibody-coated, polystyrene petri dishes in the cold. Reductions in numbers of stem cells (CFU-s) and myeloid progenitors (CFU-c) by this treatment were minimal. Of particular importance was the fact that these antibodies recognized cells in embryonic liver as well as in adults that were destined to become B lymphocytes. These observations provide new perspective on B lineage precursor heterogeneity and suggest ways of localizing and dissecting some of the earliest events that are critical to development of the humoral immune system.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface*
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology
  • Cell Differentiation
  • Clone Cells / cytology
  • Histocompatibility Antigens Class II
  • Immune Sera / pharmacology
  • Immunization, Passive
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Mice, Inbred NZB
  • Time Factors


  • Antibodies, Monoclonal
  • Antigens, Surface
  • Histocompatibility Antigens Class II
  • Immune Sera