Suppression of human T-cell mitogenesis and E-rosette formation by the monoclonal antibody OKT11A

Immunology. 1981 Dec;44(4):865-71.


OKT11A, a monoclonal anti-human T-cell antibody was studied for its in vitro effects on lymphocyte functions. At a concentration as low as 10 ng/ml, OKT11A significantly suppressed T-cell proliferation induced by OKT3, purified protein derivative (PPD), tetanus toxoid and allogeneic non-T cells. Total inhibition of proliferation was noticed at concentrations of 1-10 microgram OKT11A/ml. The antibody was only fully effective when added to stimulated cell cultures within the first 2 hr of the culturing period. OKT11A also blocked total and active sheep erythrocyte (E)-rosette formation by T lymphocytes: this activity closely paralleled the suppression of proliferative response. Quantitative studies on the binding of 125I-labelled IKT11A indicated that an average of 2 x 10(4) antibody molecules were bound per T cell. Taken together, these findings show that OKT11A recognizes a sparsely represented T-cell surface determinant that is associated with the inhibition of mitogenic responsiveness and E-rosette formation. Furthermore, our data imply that the E-rosette receptor of T cells is involved in the regulation of immune functions.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Cell Division
  • Cells, Cultured
  • Immune Tolerance
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred Strains
  • Rosette Formation
  • T-Lymphocytes / immunology*


  • Antibodies, Monoclonal