The estimation of B lymphocytes and their subpopulations in pigs. A comparison between a method using whole blood and one using Ficoll-Paque purified mononuclear cells

J Immunol Methods. 1982;50(1):33-8. doi: 10.1016/0022-1759(82)90301-5.

Abstract

This paper describes a simple method for determining the proportion of B lymphocytes and their subtypes in pigs, using washed whole blood. The method was compared with one using Ficoll-Paque purified mononuclear cells and was found to give significantly lower results (13.0 +/- 1.3% and 17.0 +/- 1.8%, mean +/- S.E., respectively). For 30 normal pigs, 13.9 +/- 6% (mean +/- S.D.) of lymphocytes in whole blood bore light chains and 12.1 +/- 5% bore IgM, while of lymphocytes in purified mononuclear cells, 17.0 +/- 6% bore light chains, 15.1 +/- 6% bore IgM, 1.4 +/- 1.4% bore IgG and 0.5 +/- 0.5% bore IgA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes* / classification
  • B-Lymphocytes* / immunology
  • Cell Separation / methods*
  • Centrifugation, Density Gradient
  • Female
  • Leukocyte Count / methods
  • Phagocytes
  • Rabbits
  • Receptors, Antigen, B-Cell
  • Swine

Substances

  • Receptors, Antigen, B-Cell