Alteration in plasma proteins and platelet functions with aging and cigarette smoking in healthy men

Thromb Haemost. 1982 Jun 28;47(3):259-64.


Blood samples were obtained on four different occasions from 18 cigarette smoking and 34 non-smoking healthy men (age 40-69) and analyzed to assess age- and smoking-associated changes in plasma proteins, blood coagulation and platelet functions. Collagen-induced platelet aggregation was significantly increased with aging in non-smokers. Significant changes in chronic smokers were increases in platelet count and fibrinogen in plasma; elevation of platelet factor-3 (PF-3) activity in platelet-poor plasma (PPP); increase in serum levels of alpha 1-antitrypsin, orosomucoid, haptoglobin and properdin factor B; and shortening of the lag period of collagen-induced platelet aggregation. Filtration of PPP through Millipore filters removed PF-3 membranes. The differences in PF-3 activities in filtered plasma were no longer significant between smokers and non-smokers. Results suggest that chronic smokers have higher levels of acute phase proteins reflecting underlying inflammatory processes, and higher levels of PF-3 activity in plasma due to liberation of PF-3 membranes from platelets.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Aging*
  • Blood Proteins / biosynthesis*
  • Collagen / pharmacology
  • Complement Factor B / biosynthesis
  • Fibrinogen / biosynthesis
  • Haptoglobins / biosynthesis
  • Humans
  • Male
  • Middle Aged
  • Orosomucoid / biosynthesis
  • Platelet Aggregation
  • Platelet Count
  • Platelet Function Tests
  • Smoking*
  • alpha 1-Antitrypsin / biosynthesis


  • Blood Proteins
  • Haptoglobins
  • Orosomucoid
  • alpha 1-Antitrypsin
  • Fibrinogen
  • Collagen
  • Complement Factor B