A novel pre-T cell line derived from acute lymphoblastic leukemia

Gan. 1982 Aug;73(4):600-5.

Abstract

A cultured cell line, P30/Ohkubo with pre-T cell phenotype, was established from an 11-year-old girl with acute lymphoblastic leukemia (ALL). According to the morphological, cytochemical and immunological analyses, P30/Ohkubo was found to have common-ALL antigen, Ia-like antigen, pan-T cell antigen, and L2 morphology with localized acid phosphatase staining, but not rosette-forming capacity with sheep erythrocytes, B1 antigen or surface immunoglobulins. Chromosome analysis revealed that P30/Ohkubo had two constant marker chromosomes [11q +, del (9)], but lacked 14q +. These findings indicate that P30/Ohkubo is a novel cultured cell line of pre-T cell phenotype. These characteristics have remained unchanged for more than 20 months since establishment of the cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Surface / analysis*
  • Cell Line
  • Child
  • Female
  • Genetic Markers
  • Histocompatibility Antigens Class II / analysis*
  • Humans
  • Karyotyping
  • Leukemia, Lymphoid / immunology*
  • Leukemia, Lymphoid / ultrastructure
  • Phenotype
  • T-Lymphocytes / immunology*

Substances

  • Antigens, Surface
  • Genetic Markers
  • Histocompatibility Antigens Class II