The preparation of protoplasts from Aspergillus fumigatus mycelium

Sabouraudia. 1980 Mar;18(1):75-7.

Abstract

Protoplasts have been prepared from the mycelium of Aspergillus funigatus, using a lytic enzyme mixture from Trichoderma harzianum. A variety of experimental conditions were investigated in order to achieve optimal conditions for protoplast production. Electron micrographs showed the preparations to be free of cell-wall. Material obtained by this procedure can be used as a source of cytoplasmic antigens for further analysis.

MeSH terms

  • Aspergillus fumigatus / ultrastructure*
  • Cell Fractionation
  • Microscopy, Electron
  • Protoplasts / ultrastructure*
  • Trichoderma / enzymology