Four methods of measuring human corneal endothelial cells from specular photomicrographs

Arch Ophthalmol. 1980 May;98(5):848-55. doi: 10.1001/archopht.1980.01020030842008.

Abstract

We measured central corneal endothelial cell density and area from contact specular photomicrographs of ten normal and ten abnormal corneas, comparing the precision, cost, and speed of four methods: a rectangle, planimeter, digitizer, and cell sizer. The rectangle, planimeter, and digitizer gave results that differed less than 10% from each other; therefore, the three methods can be used interchangeably for clinical purposes. There are statistically significant differences among the three techniques that may be important in basic research. The cell sizer gave a rapid, less precise estimate of mean cell area and cell density. The planimeter and digitizer measured individual endothelial cell size, and the latter entered data directly into a computer that printed both a copy of the endothelial mosaic and a histogram of cell size frequency, and computed cell density and mean cell area. We make the following recommendations: Count cells in a rectangle used for routine clinical measurement. use a cell sizer for rough estimation, as in an eyebank setting. Use a computerized digitizer to study individual endothelial cell size.

Publication types

  • Comparative Study

MeSH terms

  • Cell Count
  • Computers
  • Cornea / cytology*
  • Corneal Diseases / pathology
  • Costs and Cost Analysis
  • Endothelium / cytology
  • Histological Techniques*
  • Humans
  • Medical Laboratory Science / economics
  • Medical Laboratory Science / instrumentation