Abstract
Excess alpha-ketobutyrate inhibited the growth of Salmonella typhimurium LT2 by inhibiting the acetohydroxy acid synthase-catalyzed synthesis of alpha-acetolactate (a valine precursor). As a result, cells were starved for valine, and both ilvB (encoding acetohydroxy acid synthase I) and ilvGEDA (ilvG encodes acetohydroxy acid synthase II) were derepressed. The addition of valine reversed the effects of alpha-ketobutyrate.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetolactate Synthase / antagonists & inhibitors*
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Acetolactate Synthase / genetics
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Butyrates / pharmacology*
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Keto Acids / pharmacology
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Mutation
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Oxo-Acid-Lyases / antagonists & inhibitors*
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Salmonella typhimurium / drug effects
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Salmonella typhimurium / enzymology*
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Salmonella typhimurium / genetics
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Valine / pharmacology
Substances
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Butyrates
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Keto Acids
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alpha-ketobutyric acid
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Acetolactate Synthase
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Oxo-Acid-Lyases
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Valine