[Affinity modification of Escherichia coli Dna-polymerase I by 4-(N-2-chloroethyl-N-methylamino)-benzyl-gamma-amides of dTTP and dATP]

Mol Biol (Mosk). Sep-Oct 1980;14(5):1080-7.
[Article in Russian]

Abstract

The interaction of dTTP and dATP gamma-4-(N-2-chloroethyl-N-methylamino) benzylamidates with E. coli DNA-polymerase I were studied. dTTP and dATP gamma-4-(N-2-hydroxyethyl-N-methylamino) benzylamidates act as competitive inhibitors of DNA-polymerase I. Ki values for gamma-analogues of dTTP and dATP have been determined. Reactive dTTP and dATP derivatives are shown to be affinity reagent for this enzyme.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine Triphosphate / analogs & derivatives*
  • Chemical Phenomena
  • Chemistry
  • DNA Polymerase I* / antagonists & inhibitors
  • DNA-Directed DNA Polymerase*
  • Escherichia coli / enzymology*
  • Thymine Nucleotides*

Substances

  • Thymine Nucleotides
  • 4-(N-(2-chloroethyl)-N-methylamino)benzyl-gamma-amide dTTP
  • 4-(N-2-chloroethyl-N-methylamino)benzyl-gamma-amide dATP
  • Adenosine Triphosphate
  • DNA Polymerase I
  • DNA-Directed DNA Polymerase