Isolation of a yeast centromere and construction of functional small circular chromosomes

Nature. 1980 Oct 9;287(5782):504-9. doi: 10.1038/287504a0.

Abstract

The centromeric DNA (CEN3) from yeast chromosome III has been isolated on a 1.6 kilobase-pair segment of DNA located near the centromere-linked CDC10 locus of Saccharomyces cerevisiae. When present on a plasmid carrying a yeast chromosomal replicator, CEN3 enables that plasmid to function as a chromosome both mitotically and meiotically. Minichromosomes containing CEN3 are stable in mitosis and segregate as ordinary yeast chromosomes in the first and second meiotic divisions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Centromere / ultrastructure*
  • Chromosomes / ultrastructure*
  • DNA Replication
  • DNA, Circular / genetics
  • DNA, Fungal / genetics*
  • Meiosis
  • Mitosis
  • Plasmids
  • Saccharomyces cerevisiae / genetics*

Substances

  • DNA, Circular
  • DNA, Fungal