Escherichia coli K-12 clones that overproduce dam methylase are hypermutable

J Bacteriol. 1981 Jan;145(1):644-6. doi: 10.1128/jb.145.1.644-646.1981.

Abstract

A strain of Escherichia coli K-12 that overproduces dam methylase 50-fold was found to be hypermutable, and mutations which resulted in loss of excess methylase activity restored mutation frequencies to wild-type levels. These results are consistent with involvement of this deoxyribonucleic acid methylase in mismatch correction.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular
  • DNA (Cytosine-5-)-Methyltransferases / metabolism*
  • DNA Repair
  • DNA, Bacterial / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Methyltransferases / metabolism*
  • Mutation*

Substances

  • DNA, Bacterial
  • Methyltransferases
  • DNA (Cytosine-5-)-Methyltransferases